updog

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Updog provides a suite of methods for genotyping polyploids from next-generation sequencing (NGS) data. It does this while accounting for many common features of NGS data: allelic bias, overdispersion, sequencing error, and (possibly) outlying observations. It is named updog for “Using Parental Data for Offspring Genotyping” because we originally developed the method for full-sib populations, but it works now for more general populations.

The main function is flexdog, which provides many options for the distribution of the genotypes in your sample.

Also provided are:

The original updog package is now named updogAlpha and may be found here.

See also ebg, fitPoly, and TET. Our best “competitor” is probably fitPoly.

See NEWS for the latest updates on the package.

Vignettes

I’ve included many vignettes in updog, which you can access online here.

Bug Reports

If you find a bug or want an enhancement, please submit an issue here.

Installation

You can install updog from CRAN in the usual way:

install.packages("updog")

You can install the current (unstable) version of updog from Github with:

# install.packages("devtools")
devtools::install_github("dcgerard/updog")

CVXR

If you want to use the use_cvxr = TRUE option in flexdog (not generally recommended), you will need to install the CVXR package. Before I could install CVXR in Ubuntu, I had to run in the terminal

sudo apt-get install libmpfr-dev

and then run in R

install.packages("Rmpfr")

How to Cite

Please cite

Gerard, D., Ferrão L.F.V., Garcia, A.A.F., & Stephens, M. (2018). Harnessing Empirical Bayes and Mendelian Segregation for Genotyping Autopolyploids from Messy Sequencing Data. bioRxiv. doi: 10.1101/281550.

Or, using BibTex:

@article {gerard2018harnessing,
    author = {Gerard, David and Ferr{\~a}o, Luis Felipe Ventorim and Garcia, Antonio Augusto Franco and Stephens, Matthew},
    title = {Harnessing Empirical Bayes and Mendelian Segregation for Genotyping Autopolyploids from Messy Sequencing Data},
    year = {2018},
    doi = {10.1101/281550},
    publisher = {Cold Spring Harbor Laboratory},
    URL = {https://www.biorxiv.org/content/early/2018/03/16/281550},
    eprint = {https://www.biorxiv.org/content/early/2018/03/16/281550.full.pdf},
    journal = {bioRxiv}
}

Code of Conduct

Please note that this project is released with a Contributor Code of Conduct. By participating in this project you agree to abide by its terms.